[Phosphatidilinositides content study in patients with benign and malignant tumors of parotid gland].

Phosphatidilinositides content in lymphocytes, monocites and tumor tissues in 32 patients with benign and in 16 patients with malignant parotid gland tumors was investigated by the authors. It was found that the level of phosphatidilinositides content in blood lymphocytes and monocites in patients with benign tumors was significantly different from their values in patients with malignant tumors and this may be used in complex differential diagnostics.

[Efficiency of immunocorrection using cytokines in the therapy of papillomavirus infection]. / Effektivnost' immunokorrektsii tsitokinami pri lechenii papillomavirusnoi infektsii.

The data of the immunological examination and the results of treatment of 86 patients with papillomavirus infection (PVI) are presented. The multifactor suppression of cell-mediated and humoral immunity was established. The degree of immune disturbances correlated with the spread and severity of lesions. The use of systemic injections of cytokines (leukinferon and concentrated interferon) in low doses as adjuvant therapy with laser-radiosurgery led to the normalization of most immunological characteristics and the course of PVI without relapses.

[Morphological and biochemical changes in red and white muscle fibers in skeletal musculature ischemia]. / Morfobiokhimicheskie izmeneniia v krasnykh i belykh myshechnykh voloknakh pri ihemii skeletnoi muskulatury.

Structural reorganizations of red and white muscular fibres and peculiarities of changes in phosphoinositides in conditions of total ischemia of skeletal musculature were studied. On the whole morphological and biochemical changes were of the same type. However, decrease of phosphoinositides was more significant in white fibres than in red ones. In white muscular fibres deformations concerned myofibrillar apparatus while in red fibres mitochondrial apparatus was damaged.

[The effect of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, liposomes with L-DOPA and "empty" liposomes on the phospholipid composition of the murine liver]. / Vliianie 1-metil-4-fenil-1,2,3,6-tetragidropiridina, liposom s L-DOFA i "pustykh" liposom na fosfolipidnyi sostav pecheni myshei.

The effect on the liver phospholipid composition of 1-methyl-4-phenyl -1,2,3,6-tetrahydropyridine (MPTP) and liposomes containing L-DOPA and those devoid of it was studied in experiments on C57B1/6 mice. The content of separate phospholipid fractions was expressed in percentage of lipid phosphorus, in nmol lipid phosphorus per 1 mg tissue, and in nmol lipid phosphorus per 1 mg protein. It was found that the content of phosphatidylinositide and phosphatidylethanolamine changed under the effect of MPTP. Injection of liposomes stabilized the level of these phospholipids. The concentration of phosphatidylethanolamine was not restored in animals injected liposomes devoid of L-DOPA. The main changes occurring under the effect of liposomes took place in the sphyngomyelin and phosphatidylcholine fractions.

[Concentrations of phosphatidylinositols in patients different variants of neurocirculatory asthenia]. / Soderzhanie fosfatidilinozitov u bol'nykh s razlichnymi variantami neirotsirkuliatornoi distonii.

Levels of phosphatidylinosites (PI) in blood lymphocytes were measured in 59 children and adolescents (mean age 11 +/- 3.8 years) with neurocirculatory dystonia (NCD). Sex-specific minimal PI differences were determined. Contrary to childhood, in adolescence PI levels are elevated both in healthy and NCD subjects (p < 0.01). PI in controls and NCD patients differed more in arrhythmogenic, cardialgic and hypotonic NCD, less--in hypertensive variant. PI values closely correlated both with clinical course of NCD and type of neurodynamic regulation of autonomic nervous system.

[The content of phosphoinositides in the biological membranes of different types of muscle fibers]. / Soderzhanie fosfoinozitidov v biologicheskikh membranakh myshechnykh volokin razlichnykh tipov.

The content of phosphoinositids in biomembranes of slow (m.soleus) and fast (m. extensor digitorum longus) twitch muscular fibres (MF) was studied. Biochemical differences in different MF of fast and slow muscles were detected. The content of phosphotidylinosites in plasma membranes, mitochondrial and sarcoplasmic reticulum membranes of fast twitch MF was on average 1.28 times higher than in slow ones. The predominance of phosphatidylinositol-3,4,5-triphosphates in fast twitch MF over slow twitch MF was noted. The content of phosphatidylinoitol-3,4,5-triphosphates in plasma membranes, mitochondrial and sarcoplasmic reticulum membranes of slow twitch MF was 3, 2.35 and 1.25 times higher than in fast twitch MF. It was found that phosphoinositide content in biomembranes of different type MF was unequal which may be used to improve the expansion of understanding of the role of intracellular mediators in MF phenotype regulation.

[Correlation of psychoemotional state and membrane condition in patients with lung cancer in the preoperative period]. / Korreliatsiia psikhoémotsional'nykh protsessov i sostoianiia membran u bol'nykh rakom lëgkogo v predoperatsionnom periode.

The growing asthenia of the neuro-psychic system which frequently occurs in cancer patients, particularly, in the preoperative period might undermine the body's defenses and thus contribute to tumor progression. The psycho-emotional system and immunological status of lung cancer patients were corrected with a combination of measures including individual psychotherapy and tranquilizer treatment. A study of blood levels of PI, PIP, PG-F and PG-E2 as well as the use of a number of methods of psychological diagnosis showed that the psycho-emotional and immunological status improves as a result of such preoperative therapy.

[Changes in the contents of phosphoinositides in formed elements of blood and endometrial tissue in patients with hyperplastic processes and cancer of the endometrium]. / Izmenenie soderzhaniia fosfoinozitidov v formennykh élementakh krovi i tkani éndometriia u bol'nykh s giperplasticheskimi protsessami i rakom éndometriia.

The levels of phosphoinositides were measured in blood macrophages, platelets, lymphocytes, granulocytes, neutrophils, and red cells, as well as in endometrial tissue of 175 patients with glandular hyperplasia of the endometrium, atypical hyperplasia, endometrial polyps and cancer. A reliable reduction of the content of phosphatidyl inositides and phosphatidyl inositide-S-phosphates was observed in all formed elements of the blood, the most marked decrease being observed in endometrial cancer. On the contrary, the content of phosphatidyl inositide-4-phosphates and phosphatidyl inositide-4.5-diphosphates in the macrophages, lymphocytes, and granulocytes was reliably increased in the patients as against healthy women. The process of phosphatidylinositide phosphorylation run an absolutely different course in pathologically altered endometrial tissue than in comparison with that in blood cells. A new phosphatidyl inositide-signal mechanism was revealed, which is unrelated to inositol-1.4.5-triphosphate, as a result of which the potentiality of appearance of new secondary messengers actively participating in cell growth increases.

[Changes in the phosphoinositide metabolism in the blood and tissues of benign and malignant uterine tumors]. / Izmenenie fosfoinozitidnogo obmena v krovi i tkani dobrokachestvennykh i zlokachestvennykh opukholei matki.

Measurements of phosphoinositide levels in the blood, immunocompetent cells, and tumors of 105 patients with uterine myomas, 24 patients with cancer of the corpus uteri, and 17 ones with uterine sarcoma showed that the parameters of phosphoinositide metabolism in the blood of patients with tumors of the uterus reliably differed from those in healthy women. The content of phosphatidylinosites and other phosphoinositide fractions in patients with uterine myomas reliably differed from those in patients with malignant tumors of the uterus, this permitting the use of such measurements in the differential diagnosis. Phosphoinositide mechanism of development of tumors of the uterus is discussed, which is related to the "new" phosphoinositides and secondary messengers directly participating in transfer of cell growth signals.

[A clinico-morphological comparison of the ultrasonic criteria of adenomyosis]. / Kliniko-morfologicheskoe sopostavlenie ul'trazvukovykh kriteriev adenomioza.

The authors analyze the data of ultrasonic examinations in 110 patients operated on for adenomyosis and in 50 patients with this condition not exposed to surgery, in whom the diagnosis was made after comprehensive examinations. The accuracy of ultrasonic diagnosis correlated with the disease severity. Diagnostic difficulties are explained by the absence of pathognomonic echographic criteria, frequent associations with myoma, and with false echo signals emerging in the myometrium. Transabdominal and transvaginal methods may be recommended for a more precise diagnosis; it is desirable to single out several echographic criteria and by all means correlate them to clinical data. An attempt at morphologic explanation of the principal echographic criteria of adenomyosis has been made. Echo-negative structures in the myometrium emerge mainly as a result of the signal reflection from the zone of perifocal membranogenic edema of myometrial stroma developing round endometriosis focus.

[Phospholipid and glycerol levels in women's erythrocytes depending on body iron reserves]. / Soderzhanie fosfolipidov i glitserolov v éritrotsitakh zhenshchin v zavisimosti ot zapasov zheleza v organizme.

Red cell phospholipids and glycerols were measured in 18 normal by peripheral blood hemoglobin, 19 iron deficient (latent deficiency) and in 20 hypoferric anemia females. Latent and manifest iron deficiency caused a pronounced elevation in the levels of glycerols, lysophosphatidylinositols, lysophosphatidylcholines, lysophosphatidylethanolamines, phosphatidylinositol diphosphates and a drop in the phosphatidylserines in erythrocytes. This provokes serious structural and functional alterations in erythrocytic membranes of iron deficient subjects.

[The clinical picture, diagnosis and treatment of the corpus-isthmus location of uterine endometriosis interna]. / Klinika, diagnostika i lechenie korporal'no-istmicheskoi lokalizatsii vnutrennego éndometrioza matki.

The authors analyze the results of comprehensive examinations and treatment of 79 patients with corporal isthmic adenomyosis. Comprehensive examinations making use of clinical, instrumental (hysteroscopy, hysterosalpingography, echography), biochemical (measurements of blood and myometrial phosphatidylinosites), and morphologic methods are needed for a reliable diagnosis of this condition. Blood phosphatidylinosite measurements may be used as an objective test to assess the efficacy of hormonal therapy. Trental and lipostabil forte are advisable for adenomyosis therapy. If amputation of the uterus has to be resorted to, intraoperative histologic express testing of the lower edge of the uterus for endometriosis may help choose the optimal volume of surgical intervention and prevent extirpation of the uterus that is not at all neutral for the body.

[Changes in the content of phospholipids and their metabolic products in the blood and tissues of patients with endometriosis interna and cancer of the corpus uteri]. / Izmenenie soderzhaniia fosfolipidov i produktov ikh metabolizma v krovi i tkani u bol'nykh vnutrennim éndometriozom i rakom tela matki.

Levels of phospholipids, phosphoinosites, phosphatidylinosite-3-phosphates, diacylglycerols, arachidonic acid, prostaglandins E, F, J, and thromboxane A2 in patients with endometriosis and cancer of the corpus uteri were found to differ from those in healthy women. Biochemical parameters of whole blood and myometrial tissue of endometriosis patients differ significantly from those of patients with corpus uteri cancer. The authors suggest that reduction of phosphatidylinosite and phosphatidulinosite-3-phosphate levels in whole blood and their parallel increase in myometrial tissue are conducive to cellular proliferation and malignant degeneration.

[New data on the pathogenesis of endometriosis interna]. / Novye dannye o patogeneze vnutrennego éndometrioza.

Eighty-six patients with histologically verified internal endometriosis were examined using histochemical, morphometric methods, and measurements of blood and myometrial tissue phosphatidyl inosites. Blood levels of phosphatidyl inosites were found reliably decreased in the patients vs. normal subjects, whereas their levels in myometrial tissue were significantly increased. Increased counts of tissue basophils, microcirculation disorders, lymphostasis and membranogenic stromal edema development were observed round endometriosis foci. Changes in the basic substance and fibrous structures of myometrial connective tissue complex were revealed, most marked in the areas adjacent to the endometrium. A possible mechanism of the development of pathologic shifts in internal endometriosis is discussed.

[The dynamics of the rapid changes in the phospholipids of the whole blood and blood cells in patients with endometriosis interna]. / Dinamika bystrykh izmenenii fosfolipidov tsel'noi krovi i kletok krovi u bol'nykh vnutrennim éndometriozom.

Time course of rapid changes in blood phospholipids and blood cells was studied in 44 patients with endometriosis. Rapid reversible blood phospholipid changes were observed when these patients' red cells and lymphocytes were incubated with calcium chloride. Time course of rapid changes in phosphoinosites, sphyngomyelines, phosphatidylserines, phosphlatidylethanolamines of patients' red and white blood cells, whole blood, and myometrial tissue differed significantly from that in healthy women. The authors suppose that cellular proliferation processes in endometriosis are connected with phospholipid homeostasis disorders in blood cells and tissue.

[The use of blood phosphatidylinositol indices for the diagnosis of tumorous diseases of the maxillofacial area]. / Ispol'zovanie pokazatelei fosfatidilinozitov v krovi dlia diagnostiki opukholevykh zabolevanii cheliustno-litsevoi oblasti.

Phosphatidyl inositol levels were measured by flow thin-layer chromatography in red cells, platelets, lymphocytes, plasma, and whole blood of 198 patients with maxillofacial malignant and benign tumors and in 50 donors. Phosphatidyl inositol levels in the blood and its fragments were found reduced twofold in the patients with malignant tumors as against controls. Reduction of blood phosphatidyl inositol level to 185 +/- 11.0 mol of phosphatidyl inositol phosphorus per liter evidences the presence of a malignant tumor in the maxillofacial area in 97.6% of cases, this being confirmed by x-ray and histologic findings.

[Changes in phosphatidylinositol levels in the blood and its components in patients with leukemia]. / Izmenenie soderzhaniia fosfatidilinozitov v krovi i ee fragmentakh u bol'nykh leikozami.

Continuous thin-layer chromatography was used in the assay of phosphatidylinositol (PI) content in the blood and its fragments. A significant reduction in the level of these compounds was recorded in leukemia patients as compared to that in normal donors and anemia patients. The reduction of PI level in the blood may evidence that the enzymatic systems of the blood cells have partially lost their capacity for maintaining the homeostasis of these compounds. The treatment of leukemia patients with lanvis, myleran and chlorambucil improved the general condition of the patients and normalized their clinico-laboratory parameters. A significant rise of PI level in the blood and its fragments as compared to the levels before the treatment was observed in these patients. The data obtained could be used in clinical practice for complex diagnosis of leukemia as well as for the patient follow-up during the treatment with varying drugs.

[Blood level of phosphatidylinositols as a criterion of diagnosis and control of the effectiveness of surgical treatment in gastric cancer]. / Soderzhanie fosfatidilinozitov v krovi kak kriterii diagnostiki i kontrolia éffektivnosti khirurgicheskogo lecheniia raka zheludka.

In patients with gastric cancer, the content of phosphatidylinositols in the blood was significantly decreased, in average 2-fold, as compared with their level in donors. After surgical treatment in 36 patients, these indices increased and achieved a maximum value in a year. In these patients, the general state improved, there were no disease recurrences. In 44 patients after the operation, the content of phosphatidylinositols remained low, or increased insignificantly as compared with their level before the operation. In this group, 16 patients died, 10 developed a disease recurrency.

[The phosphatidylinositol content of the blood in cancer patients]. / Soderzhanie fosfatidilinozitov v krovi onkologicheskikh bol'nykh.

Phosphatidyl inosite level was measured in the whole blood, plasma, erythrocytes and platelets of patients with cancer of various sites (270), benign tumors (50), and hemoblastoses and anemia (60 cases). Phosphatidyl inosite levels in all the substrates in cancer patients, on the average, proved half the normal one while, in the benign tumor group, they only tended to decrease. The levels did not depend upon blood count.